Format

Send to

Choose Destination
Protoplasma. 2004 Jun;223(2-4):213-9. Epub 2004 Apr 21.

Ultrastructural localization of glutathione in Cucurbita pepo plants.

Author information

1
Institute of Plant Physiology, University of Graz, Schubertstrasse 51, 8010 Graz, Austria.

Abstract

Electronmicroscopic immunogold cytochemistry was used to investigate the cellular and subcellular distribution of glutathione in root and leaf cells of Styrian pumpkin (Cucurbita pepo L. subsp. pepo var. styriaca Greb.) plants. Gold particles bound to glutathione were found in various cell structures. Statistical evaluation of the gold particle density was made for different cell compartments including nuclei, mitochondria, plastids, peroxisomes, and the cytosol. In each cell type the highest level of glutathione immunoreactivity occurred in mitochondria, for which the labeling density was found to be higher in mesophyll cells of the youngest fully developed leaves (younger leaves) than in the 5th leaves (older leaves) or in root tip cells. Additionally, a statistically significant increase of gold particles bound to glutathione was observed in nuclei (22%) and the cytosol (14%) of the root cells in comparison with mesophyll cells of older (17% and 9%, respectively) and younger leaves (11% and 6%, respectively). The relevance and specificity of glutathione labeling is discussed with respect to difficulties of immunolocalization of low-molecular-weight compounds.

PMID:
15221527
DOI:
10.1007/s00709-003-0035-1
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center