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Antimicrob Agents Chemother. 2004 Jul;48(7):2708-11.

Purification and characterization of recombinant Staphylococcus haemolyticus DNA gyrase and topoisomerase IV expressed in Escherichia coli.

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1
Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, MI 48105, USA. Joel.Bronstein@pfizer.com

Abstract

The subunits of DNA gyrase and topoisomerase IV from Staphylococcus haemolyticus were expressed in Escherichia coli, purified to homogeneity, and used to reconstitute active enzymes that were sensitive to known topoisomerase inhibitors. This represents the first description of a method for isolating type II topoisomerases of a coagulase-negative staphylococcal species.

PMID:
15215131
PMCID:
PMC434177
DOI:
10.1128/AAC.48.7.2708-2711.2004
[Indexed for MEDLINE]
Free PMC Article
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