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Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1328-30. Epub 2004 Jun 22.

Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex.

Author information

1
Case Western Reserve University, Cleveland, Ohio, USA.

Abstract

Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63-amino-acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P2(1)2(1)2(1) and diffract to a resolution of 1.6 A with synchrotron radiation. Selenomethionine-containing crystals provide anomalous dispersion for phasing.

PMID:
15213406
DOI:
10.1107/S0907444904012223
[Indexed for MEDLINE]

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