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Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9740-4. Epub 2004 Jun 21.

An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues.

Author information

1
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.

Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNA genes recently found to be abnormally expressed in several types of cancer. Here, we describe a recently developed methodology for miRNA gene expression profiling based on the development of a microchip containing oligonucleotides corresponding to 245 miRNAs from human and mouse genomes. We used these microarrays to obtain highly reproducible results that revealed tissue-specific miRNA expression signatures, data that were confirmed by assessment of expression by Northern blots, real-time RT-PCR, and literature search. The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states.

PMID:
15210942
PMCID:
PMC470744
DOI:
10.1073/pnas.0403293101
[Indexed for MEDLINE]
Free PMC Article

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