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Methods Mol Biol. 2004;283:233-44.

In vitro semisynthesis and applications of C-terminally modified rab proteins.

Author information

1
Max-Planck-Institut für Molekulare Physiologie, Dortmund, Germany.

Abstract

Expressed protein ligation is a powerful tool for the generation of natively folded proteins composed of recombinantly generated and chemically synthesized polypeptides. Using this approach, we developed protocols for the production of prenylated and/or otherwise-labeled Rab GTPase. The protocols are generally applicable to most small GTPases that can be supplied with a variety of new chemical functionalities. We used semisynthetic fluorescently labeled Rab7 GTPase as a molecular probe to study protein-protein interactions with components of the prenylation machinery.

PMID:
15197315
DOI:
10.1385/1-59259-813-7:233
[Indexed for MEDLINE]

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