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FEBS Lett. 2004 Jun 18;568(1-3):65-9.

Identification of the heat shock protein 60 epitope involved in receptor binding on macrophages.

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German Diabetes Research Institute, Leibniz Institute at the Heinrich-Heine-University of Düsseldorf, D-40225 Düsseldorf, Germany.


In the present study, we identified the human heat shock protein 60 (HSP60) epitope responsible for binding to macrophages. Studies using overlapping 15- and 20-mer peptides of the human HSP60 sequence to compete with binding of HSP60 to macrophages indicated that surface binding was accounted for by the region aa481-500. Deletion mutants of HSP60, lacking the N-terminal 137, 243 or 359 amino acids, strongly inhibited HSP60 binding to macrophages. Monoclonal antibodies addressing regions aa1-200, aa335-366 or aa383-447 did not block HSP60 binding. We conclude that a single C-terminal region, aa481-500, accounts for the binding of HSP60 to macrophages.

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