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Lett Appl Microbiol. 2004;39(1):65-73.

The Bacillus secretion stress response is an indicator for alpha-amylase production levels.

Author information

1
Department of Pharmaceutical Biology, University of Groningen, Antonius Deusinglaan, Groningen, the Netherlands.

Abstract

AIMS:

Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at investigating a possible role of TepA in secretion stress management, and characterizing the intensity of the secretion stress response in relation to alpha-amylase production.

METHODS AND RESULTS:

The expression of a transcriptional htrB-lacZ gene fusion, and the levels of alpha-amylase production were monitored simultaneously using tepA mutant B. subtilis strains. TepA was shown to be dispensable for secretion stress management. Importantly, however, the levels of htrB-lacZ expression can be correlated with the levels of alpha-amylase production.

CONCLUSION:

Our observations show that the secretion stress response can serve as an indicator for alpha-amylase production levels.

SIGNIFICANCE AND IMPACT OF STUDY:

Conceivably, this stress response can be employed to monitor the biotechnological production of various secretory proteins by the Bacillus cell factory.

[Indexed for MEDLINE]
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