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Lett Appl Microbiol. 2004;39(1):19-24.

The expression of COX2 protein induced by Lactobacillus rhamnosus GG, endotoxin and lipoteichoic acid in T84 epithelial cells.

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1
The Immunopharmacological Research Group, University of Tampere, Medical School and Tampere University Hospital, Tampere, Finland.

Abstract

AIMS:

Lactobacillus rhamnosus GG (L. rhamnosus GG) possess immunomodulatory effects in the host. In the present study, the effect of L. rhamnosus GG on cyclooxygenase 2 (COX2) expression and its pharmacological control was investigated in human T84 colon epithelial cells.

METHODS AND RESULTS:

T84 cells were exposed to freeze-dried L. rhamnosus GG in vitro, and the expression of COX2 was detected by Western blot.

CONCLUSIONS:

L. rhamnosus GG induced COX2 expression in a time- and concentration-dependent manner in T84 cells. COX2 expression was inhibited by tyrosine kinase inhibitor genistein (100 micromol l(-1)), p38 mitogen-activated protein kinase (MAP kinase) inhibitor (SB203580; 1 micromol l(-1)) and dexamethasone (10 micromol l(-1)), whereas inhibitors of p42/44 MAP kinase (PD98095; 10 micromol l(-1)), protein kinase C (Ro 31-8220; 1 micromol l(-1)) and transcription factor nuclear factor kappa B (NF-kappaB) [pyrrolidinedithiocarbamate (PDTC) 100 micromol l(-1)] had practically no effect.

SIGNIFICANCE AND IMPACT OF THE STUDY:

The results provide novel information on the cellular mechanisms involved in the interaction between L. rhamnosus GG and colon epithelial cells.

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