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Curr Biol. 2004 May 25;14(10):840-50.

MAP1B is required for Netrin 1 signaling in neuronal migration and axonal guidance.

Author information

1
Developmental Neurobiology and Regeneration Unit, Institut de Recerca Biomedica de Barcelona, Barcelona Science Park and Department of Cell Biology, University of Barcelona, Barcelona 08028, Spain.

Abstract

BACKGROUND:

The signaling cascades governing neuronal migration and axonal guidance link extracellular signals to cytoskeletal components. MAP1B is a neuron-specific microtubule-associated protein implicated in the crosstalk between microtubules and actin filaments.

RESULTS:

Here we show that Netrin 1 regulates, both in vivo and in vitro, mode I MAP1B phosphorylation, which controls MAP1B activity, in a signaling pathway that depends essentially on the kinases GSK3 and CDK5. We also show that map1B-deficient neurons from the lower rhombic lip and other brain regions have reduced chemoattractive responses to Netrin 1 in vitro. Furthermore, map1B mutant mice have severe abnormalities, similar to those described in netrin 1-deficient mice, in axonal tracts and in the pontine nuclei.

CONCLUSIONS:

These data indicate that MAP1B phosphorylation is controlled by Netrin 1 and that the lack of MAP1B impairs Netrin 1-mediated chemoattraction in vitro and in vivo. Thus, MAP1B may be a downstream effector in the Netrin 1-signaling pathway.

PMID:
15186740
DOI:
10.1016/j.cub.2004.04.046
[Indexed for MEDLINE]
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