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Methods. 2004 Aug;33(4):302-11.

Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements.

Author information

1
Department of Physiology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.

Abstract

This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic beta-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other.

PMID:
15183179
DOI:
10.1016/j.ymeth.2004.01.003
[Indexed for MEDLINE]

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