Format

Send to

Choose Destination
FEBS Lett. 2004 Jun 4;567(2-3):321-6.

RACK1 inhibits the serum- and anchorage-independent growth of v-Src transformed cells.

Author information

1
Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Abstract

Cancer cells are capable of serum- and anchorage-independent growth, and focus formation on monolayers of normal cells. Previously, we showed that RACK1 inhibits c-Src kinase activity and NIH3T3 cell growth. Here, we show that RACK1 partially inhibits v-Src kinase activity, and the serum- and anchorage-independent growth of v-Src transformed cells, but has no effect on focus formation. RACK1-overexpressing v-Src cells show disassembly of podosomes, which are actin-rich structures that are distinctive to fully transformed cells. Together, our results demonstrate that RACK1 overexpression in v-Src cells partially reverses the transformed phenotype of the cells. Our results identify an endogenous inhibitor of the oncogenic Src tyrosine kinase and of cell transformation.

PMID:
15178345
DOI:
10.1016/j.febslet.2004.03.125
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center