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FEBS Lett. 2004 Jun 4;567(2-3):265-9.

NMR solution structure and position of transportan in neutral phospholipid bicelles.

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Department of Biochemistry and Biophysics, The Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden.


Transportan is a chimeric cell-penetrating peptide constructed from the peptides galanin and mastoparan, which has the ability to internalize living cells carrying a hydrophilic load. In this study, we have determined the NMR solution structure and investigated the position of transportan in neutral bicelles. The structure revealed a well-defined alpha-helix in the C-terminal mastoparan part of the peptide and a weaker tendency to form an alpha-helix in the N-terminal domain. The position of the peptide in relation to the membrane, as studied by adding paramagnetic probes, shows that the peptide lies parallel to, and in the head-group region of the membrane surface. This result is supported by amide proton secondary chemical shifts.

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