Format

Send to

Choose Destination
Methods Mol Biol. 2004;284:307-13.

Assaying cytochrome C translocation during apoptosis.

Author information

1
Cancer Immunology, Research Division, Peter MacCallum Cancer Center, Melbourne, Australia.

Abstract

Translocation of proteins from the mitochondrial intermembrane space to the cytoplasm is a critical event during apoptosis. There are several methods for assaying this event cited in the literature. In this chapter, we highlight separation of cytosolic and mitochondrial fractions of cultured cells using digitonin as the method for measuring cytochrome c release that, in our hands has been the simplest and most reproducible.

PMID:
15173625
DOI:
10.1385/1-59259-816-1:307
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center