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J Chromatogr. 1992 Mar 13;575(1):143-6.

In vivo transformation of arachidonic acid into 12-hydroxy-5,8,10,14-eicosatetraenoic acid by human nasal mucosa.

Author information

1
Department of Neurochemistry, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.

Abstract

A method for the determination of 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) by gas chromatography-mass spectrometry (GC-MS) in samples obtained from healthy subjects by nasal lavage is presented. HETEs were extracted from samples obtained by nasal lavage using C18 solid-phase cartridges. The purification of 12-HETE and 5-HETE was carried out in two consecutive steps of purification by reversed-phase high-performance liquid chromatography. Methylated and trimethylsilylated fractions were separated by GC-MS with electron-impact ionization. The production of 12-HETE by human nasal mucosa was confirmed by GC-MS.

PMID:
1517291
DOI:
10.1016/0378-4347(92)80515-r
[Indexed for MEDLINE]

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