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J Gen Virol. 2004 Jun;85(Pt 6):1675-84.

Mapping of domains responsible for nucleocapsid protein-phosphoprotein interaction of Henipaviruses.

Author information

1
Institute of Biological Sciences (Genetics), University of Malaya, 50603 Kuala Lumpur, Malaysia.

Abstract

Hendra virus (HeV) and Nipah virus (NiV) are members of a new genus, Henipavirus, in the family paramyxoviridae. Each virus encodes a phosphoprotein (P) that is significantly larger than its counterparts in other known paramyxoviruses. The interaction of this unusually large P with its nucleocapsid protein (N) was investigated in this study by using recombinant full-length and truncated proteins expressed in bacteria and a modified protein-blotting protein-overlay assay. Results from our group demonstrated that the N and P of both viruses were able to form not only homologous, but also heterologous, N-P complexes, i.e. HeV N was able to interact with NiV P and vice versa. Deletion analysis of the N and P revealed that there were at least two independent N-binding sites on P and they resided at the N and C termini, respectively. Similarly, more than one P-binding site was present on N and one of these was mapped to a 29 amino acid (aa) C-terminal region, which on its own was sufficient to interact with the extreme C-terminal 165 aa region of P.

PMID:
15166452
DOI:
10.1099/vir.0.19752-0
[Indexed for MEDLINE]

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