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Anticancer Res. 2004 Mar-Apr;24(2B):743-5.

Plant phenolics protect from bleomycin-induced oxidative stress and mutagenicity in Salmonella typhimurium TA102.

Author information

1
Department of Biochemistry-Biotechnology, University of Thessaly, Larissa, Greece.

Abstract

Antioxidants are deemed to be important against DNA damage and mutations induced by reactive oxygen species (ROS). An assay for the ability of plant phenolics to protect against mutations caused by bleomycin treatment in Salmonella typhimurium TA102 cells in concentrations up to 20 microM was developed. Caffeic acid, gallic acid, protocatechuic acid, ferulic acid and rutin hydrate in final concentrations of 0.5 to 20 microM were tested for their ability to protect TA102 cells from mutations caused by oxidative stress from bleomycin. The cut-off concentration of 20 microM was used because as a limit it is biologically meaningful, higher concentrations being unrealistic in vivo. Caffeic acid was very potent at a concentration of 0.5-20 microM. The other four antioxidants were not effective up to 20 microM. The above assay will be helpful to characterize antioxidant molecules.

PMID:
15161021
[Indexed for MEDLINE]
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