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Anticancer Res. 2004 Mar-Apr;24(2B):587-91.

The colon mitosis inhibitor pyroglutamyl-histidyl-glycine inhibits growth of non-tumorigenic colonic epithelial cells.

Author information

1
Institute of Pediatric Research, University of Oslo, The National Hospital, NO-0027 Oslo, Norway. w.h.reichelt@klinmed.uio.no

Abstract

BACKGROUND:

The colon mitosis inhibiting peptide pyroglutamyl-histidyl-glycine (pEHG) increases the expression of c-fos, fosB and egr-1 genes in the colon carcinoma cell line HT-29. However, the effect on non-tumorigenic colonic cells has not been investigated.

MATERIALS AND METHODS:

After exposure of the cell lines YAMC (from colon mucosa of Immorto mice) and IMCE (fromn Immorto-Min mouse hybrid) to pEHG, DNA-synthesis was analysed by H3-thymidine incorporation, apoptosis and necrosis by fluorescence microscopy, and cell cycle distribution by flow cytometry.

RESULTS:

pEHG inhibited DNA-synthesis with a maximal effect at 10(-8)-10(-9) M, but stimulated at 10(-4) M. It blocked cell flow through the cell cycle at GC/M after 8 h of treatment, but had no effect on apoptosis or necrosis at any concentration. A low concentration of ascorbic acid stabilised the cells, maybe as a free radical scavanger.

CONCLUSION:

pEHG inhibits flux at the G2/M transition, but has no effect on cell death.

PMID:
15160998
[Indexed for MEDLINE]
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