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Immunol Lett. 2004 May 15;93(2-3):199-204.

HSP60 and CpG-DNA-oligonucleotides differentially regulate LPS-tolerance of hepatic Kupffer cells.

Author information

1
Department of Medicine, University of Mainz, Langenbeckstr. 1, 55131 Mainz, Germany. schuchm@mail.uni-mainz.de

Abstract

BACKGROUND/AIMS:

Hepatic Kupffer cells (KC) are major regulators of the immune response to gut-derived bacterial products; uncontrolled activation of KC by bacterial components is of pathogenic relevance in alcoholic hepatitis and septic shock.

METHODS:

We examined the role of bacterial lipopolysaccharide (LPS), bacterial and autologous HSP60 and bacterial DNA, which are recognized by innate Toll-like receptors, during activation of murine KC.

RESULTS:

In cultivated KC, autologous HSP60 induced a state of LPS-hyporesponsiveness; bacterial DNA did not mitigate the response to subsequent LPS-challenge in vitro; in contrast, pre-treatment of mice with bacterial DNA even significantly increased serum TNF levels, liver function tests and mortality in a model of LPS-induced hemorrhagic liver failure.

CONCLUSION:

HSP60 and CpG-DNA differentially modulated the threshold of KC activation by LPS and might therefore contribute to the regulation of inflammatory immunity to gut-derived bacterial compounds.

PMID:
15158617
DOI:
10.1016/j.imlet.2004.03.016
[Indexed for MEDLINE]

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