Format

Send to

Choose Destination
Science. 2004 May 21;304(5674):1170-3.

Definition of a bacterial type IV secretion pathway for a DNA substrate.

Author information

1
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, JFB1.765, Houston, TX 77030, USA.

Abstract

Bacteria use conjugation systems, a subfamily of the type IV secretion systems, to transfer DNA to recipient cells. Despite 50 years of research, the architecture and mechanism of action of the channel mediating DNA transfer across the bacterial cell envelope remains obscure. By use of a sensitive, quantifiable assay termed transfer DNA immunoprecipitation (TrIP), we identify contacts between a DNA substrate (T-DNA) and 6 of 12 components of the VirB/D4 conjugation system of the phytopathogen Agrobacterium tumefaciens. Our results define the translocation pathway for a DNA substrate through a bacterial conjugation machine, specifying the contributions of each subunit of the secretory apparatus to substrate passage.

PMID:
15155952
PMCID:
PMC3882297
DOI:
10.1126/science.1095211
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center