Fungal metabolite gliotoxin inhibits assembly of the human respiratory burst NADPH oxidase

Infect Immun. 2004 Jun;72(6):3373-82. doi: 10.1128/IAI.72.6.3373-3382.2004.

Abstract

Reactive oxygen species are a critical weapon in the killing of Aspergillus fumigatus by polymorphonuclear leukocytes (PMN), as demonstrated by severe aspergillosis in chronic granulomatous disease. In the present study, A. fumigatus-produced mycotoxins (fumagillin, gliotoxin [GT], and helvolic acid) are examined for their effects on the NADPH oxidase activity in human PMN. Of these mycotoxins, only GT significantly and stoichiometrically inhibits phorbol myristate acetate (PMA)-stimulated O2- generation, while the other two toxins are ineffective. The inhibition is dependent on the disulfide bridge of GT, which interferes with oxidase activation but not catalysis of the activated oxidase. Specifically, GT inhibits PMA-stimulated events: p47phox phosphorylation, its incorporation into the cytoskeleton, and the membrane translocation of p67phox, p47phox, and p40phox, which are crucial steps in the assembly of the active NADPH oxidase. Thus, damage to p47phox phosphorylation is likely a key to inhibiting NADPH oxidase activation. GT does not inhibit the membrane translocation of Rac2. The inhibition of p47phox phosphorylation is due to the defective membrane translocation of protein kinase C (PKC) betaII rather than an effect of GT on PKC betaII activity, suggesting a failure of PKC betaII to associate with the substrate, p47phox, on the membrane. These results suggest that A. fumigatus may confront PMN by inhibiting the assembly of the NADPH oxidase with its hyphal product, GT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus fumigatus / metabolism*
  • Cell Membrane / enzymology
  • Cyclohexanes
  • Cytochrome b Group / metabolism
  • Cytosol / metabolism
  • Enzyme Activation
  • Fatty Acids, Unsaturated / metabolism
  • Fatty Acids, Unsaturated / pharmacology
  • Fusidic Acid / analogs & derivatives*
  • Fusidic Acid / metabolism
  • Fusidic Acid / pharmacology
  • Gliotoxin / pharmacology*
  • Humans
  • NADPH Oxidases / antagonists & inhibitors*
  • NADPH Oxidases / metabolism
  • Neutrophil Activation / drug effects
  • Neutrophils / enzymology*
  • Neutrophils / immunology
  • Respiratory Burst / immunology*
  • Sesquiterpenes
  • Tetradecanoylphorbol Acetate / pharmacology

Substances

  • Cyclohexanes
  • Cytochrome b Group
  • Fatty Acids, Unsaturated
  • Sesquiterpenes
  • Fusidic Acid
  • Gliotoxin
  • fumagillin
  • cytochrome b558
  • NADPH Oxidases
  • helvolic acid
  • Tetradecanoylphorbol Acetate