Abstract
Inhibitor of beta-catenin and T cell factor (ICAT) inhibits Wnt signaling by interfering with the interaction between beta-catenin and T cell factor. Here we show that ICAT(-/-) embryos exhibit malformation of the forebrain and craniofacial bones and lack the kidney. Analysis of the neuronal differentiation of embryonic stem cells revealed that Wnt3a redirects the fate of neural progenitors to a posterior character, whereas ICAT induces forebrain cells by inhibiting Wnt signaling. Furthermore, ICAT(-/-) embryonic stem cells were found to differentiate into neuronal cells possessing a posterior character. These results suggest that ICAT plays an important role in the anteriorization of neural cells by inhibiting the posteriorizing activity of Wnt signaling.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adaptor Proteins, Signal Transducing
-
Animals
-
Cell Cycle Proteins / genetics
-
Cell Cycle Proteins / metabolism*
-
Cell Differentiation*
-
Cell Lineage
-
Cells, Cultured
-
Embryo, Mammalian / cytology
-
Embryo, Mammalian / embryology
-
Embryo, Mammalian / metabolism
-
Female
-
Male
-
Mice
-
Mice, Inbred C57BL
-
Neurons / cytology*
-
Neurons / metabolism
-
Prosencephalon / cytology
-
Prosencephalon / embryology*
-
Prosencephalon / metabolism
-
Proto-Oncogene Proteins / antagonists & inhibitors*
-
Proto-Oncogene Proteins / metabolism
-
Repressor Proteins
-
Signal Transduction*
-
Stem Cells / cytology*
-
Stem Cells / metabolism
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
-
Wnt Proteins
-
Zebrafish Proteins*
Substances
-
Adaptor Proteins, Signal Transducing
-
Cell Cycle Proteins
-
Ctnnbip1 protein, mouse
-
Proto-Oncogene Proteins
-
Repressor Proteins
-
Transcription Factors
-
Wnt Proteins
-
Zebrafish Proteins