Format

Send to

Choose Destination
See comment in PubMed Commons below
Nucleic Acids Res. 2004 May 17;32(9):2707-15. Print 2004.

A three-fluorophore FRET assay for high-throughput screening of small-molecule inhibitors of ribosome assembly.

Author information

1
The Scripps Research Institute, Department of Molecular Biology, La Jolla, CA 92037, USA.

Abstract

In one of the first steps of prokaryotic ribosome assembly, the ribosomal protein S15 binds to a three-way junction in the central domain of the 16S rRNA. Binding causes a conformational change that is required for subsequent binding events. Using a novel fluorescence resonance energy transfer assay with three fluorophores, two on the RNA and one on the S15 protein, small-molecule libraries can be screened for potential inhibitors of this initial step in ribosome assembly. The employment of three fluorophores allows both the conformational change of the RNA and the binding of S15 to be monitored in a single assay.

PMID:
15148358
PMCID:
PMC419595
DOI:
10.1093/nar/gkh588
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems Icon for PubMed Central
    Loading ...
    Support Center