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Res Microbiol. 2004 May;155(4):252-8.

Transcriptional analysis of the groE and dnaK heat-shock operons of Enterococcus faecalis.

Author information

1
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610, USA.

Abstract

Enterococcus faecalis is able to survive in extremely adverse conditions, and its ability to resist stress is considered a key virulence attribute. Here, we conducted a detailed transcriptional analysis of the groE and dnaK operons of E. faecalis. The dnaK operon is comprised of four genes (hrcA-grpE-dnaK-dnaJ) preceded by two conserved CIRCE sequences. The dnaK operon is expressed from a sigmaA-type promoter located upstream of hrcA and multiple transcripts are detectable, possibly due to mRNA processing. The groE operon (groES-groEL) is transcribed as a single mRNA from a sigmaA-type promoter located immediately upstream of a CIRCE element. Induction of dnaK and groEL occurs in response to heat shock and exposure to NaCl, SDS and H(2)O(2).

PMID:
15142622
DOI:
10.1016/j.resmic.2004.02.002
[Indexed for MEDLINE]

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