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Hepatobiliary Pancreat Dis Int. 2004 May;3(2):307-10.

Detection of multidrug resistant gene 1 in pancreatic cancer.

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1
Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China. zhao8028@263.net

Abstract

BACKGROUND:

The results are conflicting in detecting P-glycoprotein (P-gp) in pancreatic cancer. The aim of this study was to detect the expression of multidrug resistant gene 1 (MDR1) in pancreatic cancer cell lines.

METHODS:

MDR1 mRNA and P-gp were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical assay (IHC) in three pancreatic cancer cell lines SW1990, CAPAN-1 and P3. P-gp functions were evaluated through the rhodamine extrusion test.

RESULTS:

Two of the three cell lines expressed MDR1 positively at different levels. The rhodamine extrusion test showed that the percentage of positive cells in MDR(+) cells was significantly lower than that in MDR1(-) cells. The results of IHC, RT-PCR and the rhodamine extrusion test were consistent with each other.

CONCLUSION:

All of these methods are reliable in the detection of MDR1 in pancreatic cancer tissue, thus providing a guide for clinical chemotherapy of pancreatic cancer.

PMID:
15138133
[Indexed for MEDLINE]
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