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[Preparation and identification of monoclonal antibodies against CD100 molecule].

[Article in Chinese]

Author information

1
Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.

Abstract

AIM:

To prepare and identify the monoclonal antibodies (mAbs) against CD100.

METHODS:

BALB/c mice were immunized with eukaryotic expressed CD100 protein. The splenocytes from immunized mice were fused with Sp2/0 cells and the positive hybridoma clones were screened by indirect ELISA and mAbs reacting to CD100 antigen were identified by indirect immunofluorescent staining and flow cytometry with human acute T lymphoblastic leukemia cell line MOLT-4, activated human PBMC, gibbon T lymphocyte cell line MLA-144 and monkey EBV-transformed B lymphoblast cell line(BLCL).

RESULTS:

Seven hybridomas secreting mAbs against CD100 molecule were obtained. All the mAbs could react to natural CD100 expressed on human MOLT-4 and activated PBMCs as well as monkey BLCL cells, and six out of them could react to gibbon MLA-144 cells.

CONCLUSION:

A set of mAbs recognizing CD100 molecule expressed on human, monkey and gibbon cells are obtained, which may provide an useful tool for studying the structure-function relationship of CD100 molecule of human, as well as gibbon and monkey.

PMID:
15132916
[Indexed for MEDLINE]

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