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J Eukaryot Microbiol. 2003 Jul-Aug;50(4):263-5.

Detection of Naegleria sp. in a thermal, acidic stream in Yellowstone National Park.

Author information

1
Thermal Biology Institute, Montana State University, Bozeman, Montana 59717, USA. umbks@montana.edu

Abstract

An initial survey of sequences of PCR-amplified portions of the 18S rRNA genes from a community DNA clone library, prepared from an algal mat in a thermal, acidic stream in Yellowstone National Park, WY, USA, revealed among other sequences, several that matched Vahlkampfia. This finding prompted further investigation using primers specific for Naegleria. Sequences from a subsequent DNA clone library, prepared from the 5.8S rRNA gene and the adjacent internal transcribed spacer (ITS) regions of the rRNA, closely matched Naegleria and formed an independent lineage within a clade containing Naegleria sturti and Naegleria niuginiensis. The sequences may represent a new Naegleria species.

PMID:
15132169
[Indexed for MEDLINE]

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