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Genome Biol. 2004;5(5):223. Epub 2004 Apr 16.

Maize DNA-sequencing strategies and genome organization.

Author information

1
Department of Agronomy and Plant Genetics, and Center for Plant and Microbial Genomics, The University of Minnesota, St Paul, MN 55108, USA. okagaoo2@tc.umn.edu

Abstract

A large amount of repetitive DNA complicates the assembly of the maize genome sequence. Genome-filtration techniques, such as methylation-filtration and high-CoT separation, enrich gene sequences in genomic libraries. These methods may provide a low-cost alternative to whole-genome sequencing for maize and other complex genomes.

PMID:
15128439
PMCID:
PMC416461
DOI:
10.1186/gb-2004-5-5-223
[Indexed for MEDLINE]
Free PMC Article
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