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J Am Chem Soc. 2004 May 12;126(18):5859-66.

X-ray magnetic circular dichroism of Pseudomonas aeruginosa nickel(II) azurin.

Author information

1
Physical Biosciences, LBNL, Cyclotron Road 1, Berkeley, California 94720, USA. funk@lbl.gov

Abstract

We show that X-ray magnetic circular dichroism (XMCD) can be employed to probe the oxidation states and other electronic structural features of nickel active sites in proteins. As a calibration standard, we have measured XMCD and X-ray absorption (XAS) spectra for the nickel(II) derivative of Pseudomonas aeruginosa azurin (NiAz). Our analysis of these spectra confirms that the electronic ground state of NiAz is high-spin (S = 1); we also find that the L(3)-centroid energy is 853.1(1) eV, the branching ratio is 0.722(4), and the magnetic moment is 1.9(4) mu(B). Density functional theory (DFT) calculations on model NiAz structures establish that orbitals 3d(x2-y2) and 3d(z2) are the two valence holes in the high-spin Ni(II) ground state, and in accord with the experimentally determined orbital magnetic moment, the DFT results also demonstrate that both holes are highly delocalized, with 3d(x2-y2) having much greater ligand character.

PMID:
15125678
DOI:
10.1021/ja036218d
[Indexed for MEDLINE]

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