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Gene. 1992 Sep 1;118(1):145-6.

An improved suicide vector for construction of chromosomal insertion mutations in bacteria.

Author information

1
Department of Microbiology, University of Queensland, St. Lucia, Australia.

Abstract

We have constructed an R6K-based suicide vector (pJP5603) that requires a trans supply of the pir-encoded pi protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying pir, it has nine unique restriction sites available for alpha-complementation cloning. Vector functionality was demonstrated in Rhodobacter sphaeroides.

PMID:
1511879
DOI:
10.1016/0378-1119(92)90263-o
[Indexed for MEDLINE]

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