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J Infect Dis. 2004 May 1;189(9):1664-75. Epub 2004 Apr 15.

Isolation and molecular characterization of a novel type 3 reovirus from a child with meningitis.

Author information

1
Department of Neurology, University of Colorado Health Science Center, Denver, Colorado 80262, USA. ken.tyler@uchsc.edu.

Abstract

Mammalian reoviruses are non-enveloped viruses that contain a segmented, double-stranded RNA genome. Reoviruses infect most mammalian species, although infection with these viruses in humans is usually asymptomatic. We report the isolation of a novel reovirus strain from a 6.5-week-old child with meningitis. Hemagglutination and neutralization assays indicated that the isolate is a serotype 3 strain, leading to the designation T3/Human/Colorado/1996 (T3C/96). Sequence analysis of the T3C/96 S1 gene segment, which encodes the viral attachment protein, sigma 1, confirmed the serotype assignment for this strain and indicated that T3C/96 is a novel reovirus isolate. T3C/96 is capable of systemic spread in newborn mice after peroral inoculation and produces lethal encephalitis. These results suggest that serotype 3 reoviruses can cause meningitis in humans.

PMID:
15116303
DOI:
10.1086/383129
[Indexed for MEDLINE]

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