Analysis of spontaneous base substitutions generated in mutator strains of Bacillus subtilis

FEMS Microbiol Lett. 2004 May 1;234(1):37-42. doi: 10.1016/j.femsle.2004.03.004.

Abstract

In the current studies, we investigated base substitutions in the Bacillus subtilis mutT, mutM, and mutY DNA error-prevention system. In the wild type strain, spontaneous mutations were mainly transitions, either G:C --> A:T or A:T --> G:C. Although both transitions and transversions were observed in mutY and mutM mutants, mutM/mutY double mutants contain strictly G:C --> T:A transversions. In the mutT strain, A:T --> C:G transversion was not observed, and over-expression of the B. subtilis mutT gene had no effect on the mutation rate in the Escherichia coli mutT strain. Using 8-oxo-dGTP-induced mutagenesis, transitions especially A:T --> G:C were predominant in the wild type and mutY strains. In contrary, transversion was high on mutY and double mutant (mutM mutY). Finally, the opuBC and yitG genes were identified from the B. subtilis chromosome as mutator genes that prevented the transition base substitutions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / physiology
  • Bacillus subtilis / genetics*
  • Bacillus subtilis / physiology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / physiology
  • DNA Glycosylases / genetics
  • DNA Glycosylases / physiology
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / isolation & purification
  • DNA-Directed RNA Polymerases / genetics
  • DNA-Formamidopyrimidine Glycosylase / genetics
  • DNA-Formamidopyrimidine Glycosylase / physiology
  • Deoxyguanine Nucleotides / pharmacology
  • Escherichia coli / genetics
  • Escherichia coli / physiology
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / physiology
  • Mutagenesis
  • Mutagenesis, Insertional
  • Mutagens / pharmacology
  • Phosphoric Monoester Hydrolases / genetics
  • Phosphoric Monoester Hydrolases / physiology
  • Point Mutation*
  • Pyrophosphatases
  • Sequence Analysis, DNA

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • DNA, Bacterial
  • Deoxyguanine Nucleotides
  • Escherichia coli Proteins
  • Mutagens
  • OpuB protein, Bacillus subtilis
  • OpuC protein, bacteria
  • 8-oxodeoxyguanosine triphosphate
  • DNA-Directed RNA Polymerases
  • RNA polymerase beta subunit
  • Phosphoric Monoester Hydrolases
  • DNA Glycosylases
  • mutY adenine glycosylase
  • DNA-Formamidopyrimidine Glycosylase
  • Pyrophosphatases
  • mutT protein, E coli