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Bioorg Med Chem Lett. 2004 May 17;14(10):2407-10.

Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation.

Author information

1
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.

Abstract

We developed a convenient method for synthesizing homogeneous DNA-protein conjugates. The method is based on expressed protein ligation of intein-fusion proteins and oligonucleotides derivatized with a cysteine. A range of cysteinyl oligonucleotides were synthesized by using a new reagent 1 and were successfully applied to expressed protein ligation to attach the oligonucleotides specifically at the C-terminus of a recombinant protein.

PMID:
15109622
DOI:
10.1016/j.bmcl.2004.03.023
[Indexed for MEDLINE]

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