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J Struct Biol. 2004 Jul;147(1):70-6.

4Pi-microscopy of the Golgi apparatus in live mammalian cells.

Author information

1
Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, High Resolution Optical Microscopy Group, 37077 Goettingen, Germany.

Abstract

We report the applicability of 4Pi-microscopy to live mammalian cells. Controlled interference of the counterpropagating wavefronts is possible despite the slight variations in cellular refractive index. Superresolved 3D-fluorescence imaging is exemplified with the first representation of the Golgi apparatus in a live cell at approximately 100 nm resolution.

PMID:
15109607
DOI:
10.1016/j.jsb.2003.10.006
[Indexed for MEDLINE]

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