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Emerg Infect Dis. 2004 Mar;10(3):413-8.

Coronaviridae and SARS-associated coronavirus strain HSR1.

Author information

1
P2-P3 Laboratories, DIBIT, San Raffaele Scientific Institute, Via Olgettina n. 58, 20132 Milan, Italy. vicenzi.elisa@hsr.it

Abstract

During the recent severe acute respiratory (SARS) outbreak, the etiologic agent was identified as a new coronavirus (CoV). We have isolated a SARS-associated CoV (SARS-CoV) strain by injecting Vero cells with a sputum specimen from an Italian patient affected by a severe pneumonia; the patient traveled from Vietnam to Italy in March 2003. Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. The full-length viral genome sequence was similar to those derived from the Hong-Kong Hotel M isolate. By using both real-time reverse transcription-polymerase chain reaction TaqMan assay and an infectivity plaque assay, we determined that approximately 360 viral genomes were required to generate a PFU. In addition, heparin (100 microg/mL) inhibited infection of Vero cells by 50%. Overall, the molecular and biologic characteristics of the strain HSR1 provide evidence that SARS-CoV forms a fourth genetic coronavirus group with distinct genomic and biologic features.

PMID:
15109406
PMCID:
PMC3322807
DOI:
10.3201/eid1003.030683
[Indexed for MEDLINE]
Free PMC Article

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