Complete nucleotide sequence of the RNA 1 of a grapevine isolate of Arabis mosaic virus

Arch Virol. 2004 May;149(5):989-95. doi: 10.1007/s00705-003-0277-4. Epub 2004 Jan 29.

Abstract

The complete nucleotide sequence of the genomic RNA 1 of the grapevine isolate NW (Neustadt an der Weinstrasse) of Arabis mosaic virus (ArMV) was determined. It is 7334 nucleotides long excluding the poly(A) tail, and contains one long open reading frame encoding a polypeptide of 2284 amino acids. The 5' and 3' non-coding regions were 227 and 252 nucleotides long respectively, and showed stretches of high identity with the corresponding 5' and 3' non-coding regions of ArMV-NW RNA 2. The analysis of the amino acid sequence of the polyprotein encoded by the RNA 1 of the ArMV-NW showed that the conserved amino acid motifs, characteristic for the viral protease co-factor, the NTP-binding protein, the cystein protease, and the RdRp core domains, were all present. Amino acid sequence comparisons between the polyproteins encoded by the RNAs 1 of ArMV-NW and other nepoviruses showed 75% identity with the GFLV-F13, and up to 36% with other nepoviruses.

Publication types

  • Comparative Study

MeSH terms

  • Genome, Viral
  • Molecular Sequence Data
  • Nepovirus / genetics*
  • Open Reading Frames
  • Plant Leaves / virology
  • RNA, Viral / chemistry
  • RNA, Viral / genetics*
  • Sequence Homology, Amino Acid
  • Vitis / virology*

Substances

  • RNA, Viral

Associated data

  • GENBANK/AY303786