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J Virol. 2004 May;78(9):4914-6.

Delivery of short hairpin RNA sequences by using a replication-competent avian retroviral vector.

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1
Laboratory of Tumor Metastasis and Angiogenesis, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA.

Abstract

While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.

PMID:
15078973
PMCID:
PMC387706
[Indexed for MEDLINE]
Free PMC Article
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