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J Intern Med. 2004 May;255(5):588-94.

Microalbuminuria and oxidative stress in essential hypertension.

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1
Department of Biochemistry of the Medical School, Hypertension Clinic, University of Valencia, Avda. Blasco Ibañez 17, 46010 Valencia, Spain.

Abstract

OBJECTIVE:

To assess the relationship between microalbuminuria and oxidative stress in mononuclear peripherals cells in essential hypertension.

METHODS:

A total of 123 hypertensive patients in absence of antihypertensive treatment were included. A 24-h ambulatory blood pressure (BP) monitoring was performed using a Spacelabs 90207 monitor, and microalbuminuria was measured in 24-h urine collections. Oxidized/reduced glutathione ratio and the content of malondialdehide and damaged base 8-oxo-2'-deoxyguanosine in genomic and mitochondrial DNA were measured in peripheral mononuclear cells.

RESULTS:

In the 29 (24%) microalbuminuric subjects, the amount of reduced glutathione was significantly lower and the ratio oxidized/reduced glutathione was significantly higher than in the normoalbuminuric subjects. In contrast, the simultaneous measurement of the levels of malondialdehide and 8-oxo-2'-deoxyguanosine from both genomic and mitochondrial DNA oxidation did not achieve statistical significance between the two groups. Subjects with the highest oxidized/reduced glutathione ratio tertile showed the highest urinary albumin excretion (UAE) (P = 0.04 for trend). In a stepwise multiple regression analysis, oxidized/reduced glutathione ratio was the main significant determinant of UAE accounting for the 9% of the variance when 24-h mean BP, age, sex, body mass index, glucose and total cholesterol were included in the model.

CONCLUSIONS:

Oxidative stress seems to be a determinant of UAE independent of BP levels even in hypertensive subjects.

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