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Nat Biotechnol. 2004 May;22(5):560-7. Epub 2004 Apr 11.

Adipose-derived adult stromal cells heal critical-size mouse calvarial defects.

Author information

1
The Department of Surgery, Stanford University School of Medicine, Stanford University, 257 Campus Drive, Stanford, California 94305, USA.

Abstract

In adults and children over two years of age, large cranial defects do not reossify successfully, posing a substantial biomedical burden. The osteogenic potential of bone marrow stromal (BMS) cells has been documented. This study investigates the in vivo osteogenic capability of adipose-derived adult stromal (ADAS) cells, BMS cells, calvarial-derived osteoblasts and dura mater cells to heal critical-size mouse calvarial defects. Implanted, apatite-coated, PLGA scaffolds seeded with ADAS or BMS cells produced significant intramembranous bone formation by 2 weeks and areas of complete bony bridging by 12 weeks as shown by X-ray analysis, histology and live micromolecular imaging. The contribution of implanted cells to new bone formation was 84-99% by chromosomal detection. These data show that ADAS cells heal critical-size skeletal defects without genetic manipulation or the addition of exogenous growth factors.

PMID:
15077117
DOI:
10.1038/nbt958
[Indexed for MEDLINE]

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