Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p

Nat Cell Biol. 2004 May;6(5):405-13. doi: 10.1038/ncb1120. Epub 2004 Apr 11.

Abstract

The GTPase Arl3p is required to recruit a second GTPase, Arl1p, to the Golgi in Saccharomyces cerevisiae. Arl1p binds to the GRIP domain, which is present in a number of long coiled-coil proteins or 'golgins'. Here we show that Arl3p is not myristoylated like most members of the Arf family, but is instead amino-terminally acetylated by the NatC complex. Targeting of Arl3p also requires a Golgi membrane protein Sys1p. The human homologues of Arl3p (Arf-related protein 1 (ARFRP1)) and Sys1p (hSys1) can be isolated in a complex after chemical cross-linking. This suggests that the targeting of ARFRP1/Arl3p to the Golgi is mediated by a direct interaction between its acetylated N terminus and Sys1p/hSys1.

MeSH terms

  • ADP-Ribosylation Factor 1 / genetics
  • ADP-Ribosylation Factor 1 / metabolism
  • ADP-Ribosylation Factors / genetics
  • ADP-Ribosylation Factors / metabolism*
  • Acetylation
  • Acetyltransferases / metabolism
  • Amino Acid Sequence
  • Animals
  • Arylamine N-Acetyltransferase / metabolism
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Endoplasmic Reticulum / metabolism
  • Golgi Apparatus / metabolism*
  • Humans
  • Macromolecular Substances
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Monomeric GTP-Binding Proteins*
  • Protein Structure, Tertiary
  • Protein Transport / physiology
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Sequence Alignment
  • Vesicular Transport Proteins*

Substances

  • Carrier Proteins
  • IMH1 protein, S cerevisiae
  • Macromolecular Substances
  • Membrane Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Sys1 protein, S cerevisiae
  • Vesicular Transport Proteins
  • Acetyltransferases
  • MAK3 protein, S cerevisiae
  • Arylamine N-Acetyltransferase
  • ADP-ribosylation factor related proteins
  • ARL1 protein, S cerevisiae
  • ADP-Ribosylation Factor 1
  • ADP-Ribosylation Factors
  • ARL3 protein, S cerevisiae
  • ARL3 protein, human
  • Monomeric GTP-Binding Proteins