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Methods Mol Biol. 2004;261:231-46.

A bacterial two-hybrid system based on transcription activation.

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1
Division of Infectious Diseases, Children's Hospital, Harvard Medical School, Boston, MA, USA.

Abstract

We describe the use of a bacterial two-hybrid system for the study of protein-protein interactions in Escherichia coli. This system is based on transcription activation and involves the synthesis of two fusion proteins within the bacterial cell whose interaction stimulates transcription of a reporter gene. Specifically, one of the fusion proteins can function as a transcription activator when its interaction partner is fused to a subunit of the bacterial RNA polymerase. This bacterial two-hybrid system has been used to study a number of interacting proteins from both prokaryotes and eukaryotes, and can be used to find interacting proteins from complex protein libraries.

PMID:
15064462
DOI:
10.1385/1-59259-762-9:231
[Indexed for MEDLINE]
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