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Methods Mol Biol. 2004;261:175-86.

Glutathione-S-transferase-fusion based assays for studying protein-protein interactions.

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1
Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA.

Abstract

Glutathione-S-transferase (GST)-fusion proteins have become an effective reagent to use in the study of protein-protein interactions. They can be produced in bacterial and mammalian cells in large quantities and purified rapidly. Given that GST can be coupled to a glutathione matrix permits its use as an effective affinity column to study interactions in vitro or to purify protein complexes in cells expressing the GST-fusion. Here we provide a technical description on the utilization of GST-fusion proteins as both a tool to studying protein-protein interactions and also as a means to purify interacting proteins.

PMID:
15064458
DOI:
10.1385/1-59259-762-9:175
[Indexed for MEDLINE]
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