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Methods Mol Med. 2004;98:9-22.

Production and characterization of recombinant human and murine TNF.

Author information

1
Biological and Technological Research, Cancer Immunotherapy and Gene Therapy Program, San Raffaele H Scientific Institute, Milan, Italy.

Abstract

Here we describe the methods for the expression of human and murine soluble tumor necrosis factor (hTNF and mTNF) in Escherichia coli cells and for their extraction, purification, and characterization. The expression and purification procedure takes about 2 wk. Human and murine TNF can be purified from crude extracts with high yields (>50 mg from 1 L of fermentation) by hydrophobic-interaction chromatography, ion-exchange chromatography, and gel-filtration chromatography. The purity and the identity of the final products can be checked by SDS-PAGE, ELISA, Western blot, analytical gel-filtration chromatography, mass spectrometry, and lipopolysaccharide assay. The biological activity of both human and murine TNF is assessed by in vitro cytolytic assays using murine L-M cells. Purified hTNF and mTNF can be used for in vitro and in vivo studies in animal models.

PMID:
15064429
DOI:
10.1385/1-59259-771-8:009
[Indexed for MEDLINE]

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