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FEBS Lett. 2004 Apr 9;563(1-3):203-6.

Identification of enolase as a plasminogen-binding protein in excretory-secretory products of Fasciola hepatica.

Author information

1
Departament de Bioquímica y Biología Molecular, Facultat de Ciències, Universitat de Valencia, C/Dr. Moliner, 50, 46100 Burjassot, Valencia, Spain.

Abstract

We have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host-parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we have identified the enolase enzyme, present in the excretory/secretory materials of F. hepatica, as a human plasminogen-binding protein. This enzyme has an apparent molecular weight of 47 kDa with pI ranging from 6.2 to 7.2. These results suggest that enolase could act as a plasminogen receptor.

PMID:
15063749
DOI:
10.1016/S0014-5793(04)00306-0
[Indexed for MEDLINE]
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