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FEMS Microbiol Lett. 2004 Apr 15;233(2):269-75.

Resuscitation of viable but non-culturable Vibrio parahaemolyticus in a minimum salt medium.

Author information

1
Department of Microbiology, Soochow University, Taipei 111, Taiwan, ROC. wonghc@scu.edu.tw

Abstract

Vibrio parahaemolyticus is food-borne pathogen prevalent in Asian countries. This work analyzes factors that influence the resuscitation of the viable but nonculturable (VBNC) state in this bacterium. The MMS-0.5% NaCl medium alone limited cell multiplication, and in this medium, resuscitation was successful when the temperature was upshifted to 25 degrees C but not 37 degrees C. Chloramphenicol inhibition experiments revealed that protein synthesis in the first 24 h of temperature upshift was critical in determining the success of the three-day resuscitation period. The VBNC state induction period and the age of the VBNC cells for successful resuscitation were strain-dependent. Results of this work facilitate further physiological and pathological study of the VBNC state in this pathogen.

PMID:
15063495
DOI:
10.1016/j.femsle.2004.02.015
[Indexed for MEDLINE]
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