Format

Send to

Choose Destination
See comment in PubMed Commons below
Mol Cell Endocrinol. 2004 Feb 12;214(1-2):149-53.

Pdcd4 inhibits growth of tumor cells by suppression of carbonic anhydrase type II.

Author information

1
Clinical Research Unit for Gastrointestinal Endocrinology, University of Marburg, Baldingerstrasse, 35033 Marburg, Germany.

Abstract

To identify new genes that are upregulated during apoptosis we previously cloned rat pdcd4. While the role of pdcd4 is still unclear it seems to possess a tumor suppressor activity. Pdcd4 directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. In the present study, we show that pdcd4 suppresses carbonic anhydrase type II protein expression in HEK293 and Bon-1 carcinoid cells. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Similar to pdcd4, carbonic anhydrase inhibitor ethoxyzolamide reduces growth of several endocrine tumor cell lines. Thus, the translation inhibitor pdcd4 represses endocrine tumor cell growth by suppression of carbonic anhydase II. Furthermore, carbonic anhydrase inhibitors might represent promising tools for anti-endocrine tumor treatment.

PMID:
15062553
DOI:
10.1016/j.mce.2003.10.058
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center