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J Antimicrob Chemother. 2004 May;53(5):739-42. Epub 2004 Mar 31.

A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp.

Author information

1
Servicio de Microbiología, Hospital Universitario de Valme, Ctra Cádiz s/n, Sevilla 41014, Spain.

Abstract

OBJECTIVE:

The activity of voriconazole against Aspergillus spp. (n = 77) was tested by the Etest, disc diffusion and the NCCLS M38-A methods.

METHODS:

Four Rhizomucor spp. isolates were included to study the suitability of the three susceptibility testing methods to detect isolates resistant to voriconazole. The disc diffusion method performed on Mueller-Hinton agar (Difco) supplemented with 2% glucose and Methylene Blue (0.5 mg/L) gave zone diameters with very clear border edges.

RESULTS:

The disc diffusion method showed excellent correlation with the Etest and the NCCLS methods.

CONCLUSION:

On the basis of our results, it appears that the disc diffusion test is a useful method for testing the activity of voriconazole against Aspergillus spp.

PMID:
15056643
DOI:
10.1093/jac/dkh172
[Indexed for MEDLINE]

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