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Can J Microbiol. 2001 Jan;47(1):41-8.

Competitiveness in root colonization by Pseudomonas putida requires the rpoS gene.

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Department of Biology, Utah State University, Logan, UT 84522-5305, USA.


The rpoS gene in Pseudomonas putida was essential for plant root colonization under competitive conditions from other microbes. The RpoS- mutant survived less well than the wild-type strain in culture medium, and unlike the wild-type, failed to colonize the roots in a peat matrix containing an established diverse microflora. The RpoS-deficient P. putida isolate was generated by insertion of a glucuronidase-npt cassette into the rpoS gene. The RpoS mutant had dose-dependent increased sensitivity to oxidative stress and produced Mn-superoxide dismutase activity earlier than the parent. While extracts from wild-type P. putida stationary-phase cells contained three isozymes of catalase (CatA, CatB, and CatC), the sigma38-deficient P. putida lacked CatB. These results are consistent with previous findings that CatB is induced in stationary-phase.

[Indexed for MEDLINE]

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