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Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):5164-9. Epub 2004 Mar 26.

Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2.

Author information

1
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.

Abstract

Agrobacterium tumefaciens transfers DNA to plant cells as a single-stranded DNA molecule (the T-strand) covalently linked to VirD2 protein. VirD2 contains nuclear localization signal sequences that presumably help direct the T-strand to the plant nucleus. We identified a tomato cDNA clone, DIG3, that encodes a protein that interacts with the C-terminal region of VirD2. DIG3 encodes an enzymatically active type 2C serine/threonine protein phosphatase. Overexpression of DIG3 in tobacco BY-2 protoplasts inhibited nuclear import of a beta-glucuronidase-VirD2 nuclear localization signal fusion protein. Thus, DIG3 may be involved in nuclear import of the VirD2 protein and, consequently, the VirD2/transferred DNA complex.

PMID:
15047887
PMCID:
PMC387391
DOI:
10.1073/pnas.0300084101
[Indexed for MEDLINE]
Free PMC Article

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