A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought- and low-temperature stress tolerance in tobacco by gene transfer

Plant Cell Physiol. 2004 Mar;45(3):346-50. doi: 10.1093/pcp/pch037.

Abstract

The transcription factor DREB1A/CBF3 specifically interacts with the dehydration responsive element (DRE/CRT) and induces expression of genes involved in environmental stress tolerance in Arabidopsis: Overexpression of DREB1A improved drought- and low-temperature stress tolerance in tobacco. The stress-inducible rd29A promoter minimized the negative effects on the plant growth in tobacco. Furthermore, we detected overexpression of stress-inducible target genes of DREB1A in tobacco. These results indicate that a combination of the rd29A promoter and DREB1A is useful for improvement of various kinds of transgenic plants that are tolerant to environmental stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acclimatization / genetics*
  • Acclimatization / physiology
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Cold Temperature
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Disasters
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant
  • Molecular Sequence Data
  • Nicotiana / genetics*
  • Nicotiana / growth & development
  • Plant Proteins / genetics
  • Plants, Genetically Modified
  • Promoter Regions, Genetic / genetics*
  • Sequence Analysis, DNA
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transcription Factors*

Substances

  • Arabidopsis Proteins
  • DNA, Complementary
  • DREB1A protein, Arabidopsis
  • Plant Proteins
  • Trans-Activators
  • Transcription Factors
  • dehydrin proteins, plant

Associated data

  • GENBANK/AB049335
  • GENBANK/AB049336
  • GENBANK/AB049337
  • GENBANK/AB049338