Influence of air on polybutadiene used in the preparation of stationary phases for high-performance liquid chromatography

J Chromatogr A. 2004 Mar 19;1030(1-2):231-6. doi: 10.1016/j.chroma.2003.12.035.

Abstract

A 100 ml bottle of polybutadiene, PBD, was repeatedly exposed to air over a period of 6 months. Samples were taken at time zero (PBD-0), after 3 months (PBD-3) and 6 months (PBD-6). These samples were sorbed onto HPLC silica by an open-air solution-evaporation procedure, which involved exposure to the atmosphere for 6 days. Portions of the three sets of samples were used to compare self-immobilization and the effects of 100 degrees C thermal treatments in air or nitrogen on HPLC performance of the resulting phases. It is concluded that self-immobilization is enhanced by prior exposure of sorbed PBD to air and subsequent heating at 100 degrees C further enhances column performance. The best performance (10(5) plates m(-1)) resulted from 4 h heating of PBD-6 material in nitrogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Air*
  • Butadienes / chemistry*
  • Chromatography, Gel / methods*
  • Chromatography, High Pressure Liquid / methods*
  • Elastomers
  • Polymers / chemistry*

Substances

  • Butadienes
  • Elastomers
  • Polymers
  • polybutadiene